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Large-performance liquid chromatography (HPLC) is usually a broad analytical chemistry technique used to independent compounds in a chemical mixture. These separations utilize the tension-driven circulation of the mobile period through a column full of a stationary phase.
Mass spectra contain info regarding the elemental and isotopic composition of analytes, which yields higher detection specificity and is useful for structural elucidation.
Find out more about our HPLC detectors, and check out our HPLC detector range guideline to search out the correct detector to suit your needs.
Agilent HPLC lamps are produced within an ISO 9001 certified natural environment and are thoroughly traceable through each individual stage in the generation process. Every lamp is independently tested and Accredited which has a Certificate of research to ensure it fulfills the demanding Agilent efficiency requirements.
Here are some of The key aspects to consider When selecting a detector for HPLC and UHPLC analyses:
Instrument capabilities made available depend upon instrument configuration. Attributes outlined right here might not be current in some configurations.
Peak purity: Peak purity, or peak homogeneity, is usually expressed being a peak purity index or purity angle attained by evaluating the UV spectra of the upslope for check here the downslope in the chromatographic peak.
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HPLC lamps are gentle sources suitable for HPLC detectors. Agilent HPLC detector lamps are vital things, together with the optical unit, and provide ideal depth, sensitivity, and balance. Agilent HPLC lamps ended up formulated along with Agilent detector modules for maximum compatibility.
Tandem gradient operates section into two principal areas: Pump just one provides the analytical gradient to column a single though pump two reconditions. Pump a person then delivers the analytical gradient to column two though pump two reconditions column 1.
Fluorescence detectors are exceptionally selective for fluorogenic compounds, and excitation and emission are tunable for a selected course of fluorophore.
The linearity in the detector refers to its ability to offer a reaction that’s proportional towards the focus of your analyte. A linear response is important for accurate quantification of analytes.
Solitary-Use chromatography methods from Sartorius together with resolute columns and membrane adsorbers hplc detectors types provide unparalleled overall flexibility for multi-item manufacturing at pilot/ scientific scale.